FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7526377
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521280.6926041573522027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353980.4703192518790914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279950.3719585133723703No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176950.23510647951863162No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG144460.1919382991311756No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG106840.1419540902614897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCTC59350.055.1460841
GTCCCAA58550.053.358681
GTATCAA322050.051.1279641
GGTATCA218200.050.4805831
CCAACCA71400.044.2536164
TCCCAAC74650.043.2855222
CCAGGAC75750.041.6285068
CAACGCA430550.039.857735
AACGCAG436400.039.3889926
ATCAACG437250.039.3587343
TCAACGC439050.039.1684464
TATCAAC439950.038.941352
GTCTCGC23300.038.8204271
ACGCAGA469450.036.5394177
CGCAGAG472700.036.3504038
TCTGTCG25800.035.9751438
ACCAGGA90600.035.200027
TCTCGCT26100.034.8837852
GCAGAGT501250.034.4105389
CTCGCTC25700.033.3427963
Produced by FastQC (version 0.11.5)