FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9227036
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193750.20998075655064094No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA184730.2002051362972898No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG146990.15930359435034175No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA119320.12931563288579345No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA110250.11948582405010666No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG101700.11021957647071062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGTT74450.052.9709853
CCCGTTG75850.051.9932754
GGTATCA114000.050.7130741
GGCCCGT78350.050.6376652
GGGCCCG79150.050.2728771
GTATCAA218050.049.1538121
GTCTCGC27200.042.6406941
TCTGTCG30250.042.455018
ATCAACG262350.040.1084563
CAACGCA263650.039.7534145
AACGCAG265950.039.3688626
TCAACGC267300.039.2766954
TCTCGCT29400.038.8455052
CGCAGAG277050.037.7277838
CGTTGGG103400.037.7204446
ACGCAGA281350.037.1920247
CTCGCTC29800.036.7274483
CGCTCTG28700.035.8558925
TATCAAC301900.035.346482
TTATCAC57400.034.4045144
Produced by FastQC (version 0.11.5)