FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004912997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004912997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9189910
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236540.25739098641880065No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA174500.18988216424317542No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG150340.16359246173248704No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA121450.13215581001337337No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG101820.11079542672343909No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA101510.11045810024254861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109550.053.8416181
GCCCGTT69250.052.9557153
CCCGTTG71600.051.302754
GTATCAA216650.049.219081
GGCCCGT75450.048.289562
GGGCCCG76100.047.8770981
GTCTCGC27500.046.8242841
CAACGCA256700.040.963865
ATCAACG258750.040.821013
AACGCAG260350.040.45756
TCTGTCG30250.040.411058
TCTCGCT30600.040.3353652
TCAACGC262400.040.2775574
CGTTGGG94750.038.767676
CGCAGAG272400.038.5153128
ACGCAGA277000.037.9828267
TTATCAC54050.035.7919124
CGCTCTG30500.035.6052675
TATCAAC300400.035.437792
CCACCGA43250.035.1976736-37
Produced by FastQC (version 0.11.5)