FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4806853
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348890.7258179103875239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217190.45183407938624287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144480.3005708724606307No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120260.250184476205118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107600.059.5073471
GTATCAA210650.055.7360571
TCTGTCG31150.052.143838
GTCTCGC28100.048.2835461
CTCGCTC27250.046.2956283
TATCAAC265700.044.232982
CAACGCA264600.044.1423955
ATCAACG266150.043.9122433
TCAACGC266950.043.7342454
AACGCAG268350.043.7011036
TCTCGCT30150.043.6189882
ACGCAGA285450.040.9581077
CGCAGAG288550.040.5378538
TCGCTCT33050.040.5101244
CTGTCGC39350.040.3705679
CGCTCTG31400.039.414785
GCAGAGT307800.037.925279
GTACTTT351150.037.4685331
GTCTCAC45300.035.4679571
TACTTTT371650.035.305722
Produced by FastQC (version 0.11.5)