FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7723830
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288960.37411491449190365No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG128190.16596688430480733No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81600.10564706887645119No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT77760.10067544210579467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74150.052.045251
GTACTTT76800.050.171991
TCTGTCG28200.047.432588
GTATCAA159450.042.0287931
GTCTCGC30450.041.2043151
ATCAACG157250.041.144973
TCAACGC161950.039.914434
CAACGCA164000.039.379755
TCTCGCT32400.039.094272
GTACGGG308250.038.831861
ACGCAGA171600.037.587357
AACGCAG172750.037.407176
CGCAGAG173800.037.0101478
CGCTCTG32100.034.82895
TACGGGG113150.034.0564162
CTCGCTC35650.033.1949543
TACGGGT31700.032.828972
ACGGGAC23950.032.5270233
GCAGAGT206450.031.1862079
ACGGGGA38150.031.1755373
Produced by FastQC (version 0.11.5)