FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7688421
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA309040.40195509585128075No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG133470.1735987142223351No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT83270.10830572363298005No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82640.10748630960765546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73850.053.1402851
GTACTTT77700.050.5837331
TCTGTCG28600.046.1524478
GTATCAA161250.043.4386181
ATCAACG157850.042.71963
GTCTCGC29350.042.139141
TCAACGC161200.041.685624
CAACGCA163750.041.0727845
TCTCGCT31250.040.531622
AACGCAG170150.039.630926
CGCAGAG170150.039.5219578
ACGCAGA170500.039.5144357
GTACGGG302100.038.4398731
CGCTCTG30000.037.8603825
CTCGCTC33650.034.8128743
ACGGGGC36300.033.7457663
TACGGGG115900.033.1446532
GGCTAGA24650.033.0471571
GCAGAGT203150.032.984029
ACGGGAC24550.031.0039623
Produced by FastQC (version 0.11.5)