FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7632446
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA299960.39300638353681117No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG132500.17360096619091706No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85130.11153698303270013No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT79400.10402956011742501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT80350.053.8025931
GGTATCA77000.050.5830761
TCTGTCG29350.044.7621278
GTATCAA155750.040.9280851
GTCTCGC30950.039.1944161
ATCAACG157700.039.0324673
TCTCGCT31800.038.339042
TCAACGC160650.038.0573234
CAACGCA161700.037.9941025
GTACGGG299600.037.0360371
AACGCAG169450.036.067896
ACGCAGA170850.036.05127
CGCAGAG171050.035.8660168
ACGGGGC33350.035.4874343
CTCGCTC35500.033.0031363
GGCTAGA26100.032.5799371
CGCTCTG33050.032.031295
ACGGGAC22900.031.6841243
TACGGGT31550.031.6682762
TACGGGA158750.031.2440132
Produced by FastQC (version 0.11.5)