FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7847319
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT571530.728312433838869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398540.5078677188986456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288950.3682149279263402No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281920.35925645433809944No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG142180.18118289826117684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98470.1254823462637367No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92270.11758155874636932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142550.076.032731
GTATCAA319850.052.8048861
TCTGTCG28050.047.9312488
AAGTACG8700.045.9123461
ATCAACG401600.045.007433
TCAACGC409500.044.2263344
CAACGCA419200.043.256435
AACGCAG434150.041.8614776
TCTCGCT31850.041.6567732
TATCAAC455100.039.6511462
ACTGCCT65600.038.9876751
GTCTCGC30350.038.8938061
ACTGCCC69500.038.8586161
ACGCAGA468750.038.7588547
CGCAGAG471650.038.558388
GTACTTT591100.035.7241171
GCAGAGT510000.035.6472749
CTCGCTC34800.035.5610283
CGCTCTG33850.035.5017435
ACTGCCG7250.035.3593561
Produced by FastQC (version 0.11.5)