FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7847319
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA375620.4786602914957325No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG129920.16555972810586647No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT83750.10672434751282571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70550.052.9906041
GTACTTT79900.048.274961
TCTGTCG30100.046.9226348
GTATCAA152800.042.3698351
ATCAACG153350.041.057243
TCAACGC156950.040.0789644
CAACGCA158100.039.8625075
GTCTCGC31550.038.557591
GTACGGG300150.038.157011
AACGCAG167350.037.623476
ACGCAGA169050.037.2451257
TCTCGCT31750.037.193542
CGCAGAG169800.037.04598
ACGGGAC24750.035.724753
CGCTCTG29550.034.3408165
ACGGGGC35350.032.902193
TACGGGG112250.031.6135272
CTCGCTC35300.031.6039413
GTCTCAC43100.031.5292471
TACGGGA167400.031.3013732
Produced by FastQC (version 0.11.5)