FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12198087
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA407470.3340441825017316No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG215200.17642110603080632No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT140440.11513280730002992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG56550.050.6792378
GTCTCGC47700.049.869991
TCTCGCT51650.046.060282
CTCGCTC48850.045.900483
CGCTCTG50600.043.1384855
GTACTTT98150.041.020031
ACGGGAC28350.040.2799223
GTACGGG440850.038.702351
ACGGGGC49250.038.6441883
GGTATCA123000.037.2291641
GTATCAA234100.036.682881
GTCTCAC86600.036.6708531
CAACGCA228950.035.6420635
ATCAACG233000.035.2004973
TCAACGC234800.034.930934
TACGGGG160600.033.7372552
GGCTAGA45050.033.6622431
CGCAGAG245850.033.3026928
ACGCAGA247250.033.136277
AACGCAG249650.032.8896986
Produced by FastQC (version 0.11.5)