FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12134019
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA432270.3562463516828184No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG224710.18519008417573765No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT146570.12079262443877828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG53750.051.991368
GTCTCGC50450.047.618221
CTCGCTC49700.045.4677543
TCTCGCT54350.044.9702952
GGTATCA112550.041.791121
GTACTTT101150.041.38661
CGCTCTG53050.041.3648645
ACGGGAC28850.040.400483
GTACGGG439300.039.457571
GTATCAA228200.038.5133931
GTCTCAC81750.038.0423131
CAACGCA224450.037.782495
ATCAACG225100.037.6721463
TCAACGC227900.037.158344
ACGGGGC51200.036.818463
CGCAGAG237600.035.684938
ACGCAGA238850.035.5268827
AACGCAG241300.035.166316
ACGGGTC10250.034.809973
GGCTAGA48550.034.4167671
Produced by FastQC (version 0.11.5)