FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186085
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790710.6488630269688748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542500.4451798916551132No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445220.36535113615242304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436070.35784257208118936No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG214600.17610249723352495No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187800.15411020028171477No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT125330.10284681257352135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222800.066.5875551
GTATCAA467000.051.1429521
AAGTACG7850.049.3545341
TCTGTCG48450.046.0458
ATCAACG581250.041.7340433
GTCTCGC43050.041.6751021
TCAACGC591250.041.0281834
CAACGCA598950.040.565615
GTACTTT966700.040.1210631
TCTCGCT49000.039.8281822
CTCGCTC45450.039.7971883
AACGCAG624550.039.0250786
CGCTCTG47650.036.9565665
ACGCAGA672250.036.300277
TATCAAC668850.036.276992
CGCAGAG675900.036.095448
ACGGGAC27650.035.9363563
GTACGGG409700.035.7310451
GGCTAGA42600.035.6791041
ACTTTTT1119100.035.6007583
Produced by FastQC (version 0.11.5)