FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186085
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA496540.4074647435989492No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG215790.17707902086683294No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT144960.11895534948262712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG51750.051.268458
GTCTCGC49500.046.7640881
TCTCGCT53500.042.8241162
CTCGCTC51200.041.6178253
GTACTTT88900.039.8589321
ACGGGAC28050.039.781383
GTACGGG426900.039.082991
CGCTCTG52500.038.8922355
GGTATCA111200.038.1106641
GTATCAA219150.037.7820781
CAACGCA215900.036.757215
ACGGGGC50250.036.380573
GGCTAGA48550.036.30951
ATCAACG219450.036.2700423
TCAACGC219400.036.1167264
GTCTCAC77750.035.8035051
CGCAGAG230300.034.458048
ACGCAGA230200.034.4472247
TACGGGG162200.034.3978962
AACGCAG230200.034.3701556
Produced by FastQC (version 0.11.5)