FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10630226
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT956320.8996233946484298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551630.5189259381691415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394370.3709892903499888No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140410.1320856207572633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148200.079.2180561
GTATCAA357900.065.2231751
ATCAACG447900.053.3396033
TCAACGC454200.052.637324
CAACGCA461300.051.8390925
TCTGTCG50050.050.64078
AACGCAG474600.050.4083676
TATCAAC504000.047.5676882
ACGCAGA509500.046.8036587
CGCAGAG511600.046.576438
GTCTCGC48600.043.9545171
CTCGCTC48650.043.0531653
CGCTCTG46050.042.2513855
GCAGAGT565750.042.1289529
TCTCGCT54000.042.0934872
TGGTATC53700.034.7937052
CTGTCGC74050.034.1474349
GTGGTAT59300.032.1100231
AGAGTAC558350.031.60522510-11
TGCGGAC6050.031.4694259
Produced by FastQC (version 0.11.5)