FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10618432
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT944090.889104907391223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT545360.5135974878400125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390400.36766257014218295No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137830.12980259232248229No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148250.078.363261
GTATCAA365550.065.254181
ATCAACG454650.054.0923463
TCAACGC460750.053.389124
CAACGCA467350.052.544295
TCTGTCG51250.051.195658
AACGCAG481700.051.010756
TATCAAC506700.048.841122
ACGCAGA517500.047.4588977
CGCAGAG519900.047.2398158
GTCTCGC48000.046.1242261
CTCGCTC48600.045.423993
CGCTCTG47150.045.1787955
TCTCGCT53550.043.2252732
GCAGAGT577800.042.5268149
TGCGGAC5400.038.562459
CTGTCGC77750.033.976059
GTACGGG52650.033.45961
TGGTATC56400.033.4447362
GTGGTAT59900.031.9931091
Produced by FastQC (version 0.11.5)