FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10618432
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG51950.047.4892128
GTCTCGC50300.045.277661
CTCGCTC47500.044.322923
TCTCGCT52900.042.721112
CGCTCTG46350.042.472045
TGCGGAC6150.033.831349
CTGTCGC77100.032.691729
GCAGCGC9050.031.530088
TGTCGCG2151.2187229E-1030.4276812
GTACGGG59400.029.631831
GGCTAGA51750.029.4159471
TCGCTCT81650.029.0630744
CAACGCA40700.028.787025
TCAACGC41300.028.512814
GTCTCAC98000.027.9722921
GCTCTGT141150.027.6727126
CGCAGAG44550.026.421048
ATCAACG45600.026.2150043
ACGCAGA45900.025.6461267
TACGGGA33950.023.9991252
Produced by FastQC (version 0.11.5)