FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665041
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT933780.8755521896259002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT554980.520373058106387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375610.35218805066009595No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121890.11428929340262266No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107060.10038404915649177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156500.082.323411
GTATCAA366600.063.9608761
ATCAACG459900.051.2572633
TCTGTCG50000.050.6839688
TCAACGC466150.050.570024
CAACGCA472650.049.8165055
AACGCAG488900.048.233046
TATCAAC513900.045.9869882
GTCTCGC50300.045.86971
CTCGCTC49700.044.8930633
ACGCAGA526450.044.724937
CGCAGAG527800.044.5992668
TCTCGCT53900.044.154562
CGCTCTG49150.042.6045765
GCAGAGT585900.040.1969459
TGCGGAC5100.039.6588179
TGGTATC55100.034.986282
CTGTCGC76050.034.0267949
GTGGTAT60350.033.2916681
GTACGGG55850.031.1704161
Produced by FastQC (version 0.11.5)