FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665041
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG51200.042.8839078
GTCTCGC47100.042.4579851
CTCGCTC44550.041.5588383
TCTCGCT49100.040.0039632
CGCTCTG44350.038.535725
CAACGCA41800.031.8006785
TCAACGC43500.030.8307344
CTGTCGC75650.030.2010869
CGCAGAG44050.030.1762128
ACGCAGA44650.029.7702927
GTACGGG61800.029.7662141
ATCAACG45600.029.6706123
GGCTAGA50100.029.6109731
TGCGGAC5950.028.9235659
GCAGCGC10850.027.8935038
AACGCAG49150.027.0450086
GTCTCAC99150.026.9320981
TCGCTCT77000.026.8196544
GCTCTGT135100.025.7837096
ACGGGAC7400.024.0572533
Produced by FastQC (version 0.11.5)