FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10179791
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT599910.589314652923621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376860.370204064110943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213770.2099944880990189No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201180.19762684715236295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC4950.072.118261
GCGTACT5200.061.7859421
TCTGTCG91250.060.110218
GGTATCA188550.059.3555761
GTCTCGC84750.058.9710431
GCGTAAG12150.058.763031
GTATCAA372250.056.2769551
CTCGCTC84050.056.1312683
CGCTCTG80050.055.8889435
CGTAAGA4800.055.7756842
CGTAACG2250.055.5277982
TCTCGCT90500.054.432142
TCGCTCT95550.053.4227184
CGTACTT5200.052.6293642
GCGCAGA8000.050.5729371
TCACTCT150500.050.006154
GTCTCAC136200.048.9260521
GGCTAGA72850.047.7777371
CGTACGT1152.764864E-1046.560572
TATCAAC462700.045.170232
Produced by FastQC (version 0.11.5)