FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10179791
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117320.11524794566018105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG82450.058.6219028
GTCTCGC79350.053.8121951
CTCGCTC76850.052.3927883
CGCTCTG78350.049.8720635
TCTCGCT86700.049.5273172
TCGCTCT90650.048.0252884
TCACTCT147100.044.27194
GTCTCAC133600.043.178591
GGCTAGA72700.041.9648931
GTATCAA191400.041.480361
CTGTCGC117700.040.762959
GGTATCA116450.039.4258461
TCTGTCA134750.038.1193058
TAGAGTG75950.037.7438284
TGCGGAC7900.037.628039
GTACGGG437600.037.2915081
GCTAGAG79050.036.8655622
TCTCACT159900.036.859712
GCTCTGT192050.036.3418776
CTAGAGT83300.035.841443
Produced by FastQC (version 0.11.5)