FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10170810
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596690.5866691050172012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375260.36895783128384074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216020.21239213002700866No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200310.19694596595551386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC4100.075.466781
GCGTACT5600.066.940481
TCTGTCG92550.062.8718268
GGTATCA192400.061.3891751
GTCTCGC88500.058.8303071
CGTAACG2150.058.118872
GCGTAAG13050.057.9067961
CTCGCTC82700.056.8405083
TCTCGCT89200.055.9004752
GTATCAA369200.055.828061
CGCTCTG81450.055.3731885
TCGCTCT96900.054.219854
CGTACTT6400.049.2756352
GTCTCAC129750.048.2900161
GGCTAGA76350.047.1501051
TCACTCT149150.047.07394
TGCGGAC7900.046.6936959
CTGTCGC127650.045.863589
TAGAGTG79750.045.5114364
TATCAAC458700.044.883192
Produced by FastQC (version 0.11.5)