FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10170810
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124360.12227148083584297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC84650.056.615411
CTCGCTC82200.055.6340833
TCTGTCG86700.055.6243178
TCTCGCT90000.053.2570152
CGCTCTG81750.052.8149265
TCGCTCT97000.051.623584
TCACTCT144450.045.287984
GTCTCAC132950.044.723751
GTATCAA187950.041.823051
GGTATCA115650.040.7198451
GGCTAGA72700.040.5670851
TGCGGAC8100.040.3746269
TCTGTCA133300.039.702958
CTGTCGC124300.038.077889
TCTCACT157200.037.4891362
TAGAGTG77900.037.255484
GCTCTGT195900.037.2476356
GTACGGG430000.037.224871
CTCACTC152050.036.7253233
TCTGTTG87850.035.740048
Produced by FastQC (version 0.11.5)