FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10135088
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586950.5791266933252084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370390.3654531662675253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212930.21009191040077796No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197500.1948675729307925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC4300.083.036491
TCTGTCG92150.063.467318
GGTATCA187950.061.773351
GCGTAAG12450.061.660431
GTCTCGC87800.061.271731
GCGTACT5650.061.0893861
CTCGCTC86700.058.335153
TCTCGCT92250.058.115092
CGCTCTG82200.056.750325
GTATCAA364150.055.8080141
CGTACCG1300.054.924792
CGTAACG2400.054.5433652
CGTACTT5900.054.4593282
TCGCTCT101450.054.3698584
TCACTCT151750.048.8562164
GTCTCAC138650.048.3286551
GCGCAGA7650.048.2299081
CTGTCGC124850.046.7492339
GGCTAGA75300.045.916351
TATCAAC448200.045.283542
Produced by FastQC (version 0.11.5)