FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10135088
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120730.1191208206578966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG87250.058.8725058
GTCTCGC82950.057.068721
CTCGCTC79550.056.4486163
TCTCGCT87850.053.9588932
CGCTCTG78950.052.659395
TCGCTCT92550.051.92614
TCACTCT146450.045.201894
GGCTAGA70300.044.666311
GTCTCAC133350.044.5526921
CTGTCGC123150.041.2746129
GTATCAA192450.041.253881
GGTATCA119550.040.5424041
TAGAGTG75000.038.541254
GCTAGAG76500.038.32942
TCTGTCA134600.037.543798
TCTCACT156950.037.402652
GTACGGG438250.036.8968051
GCTCTGT196850.036.6673626
CTAGAGT79650.036.5148543
CTCACTC155400.035.402773
Produced by FastQC (version 0.11.5)