FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10275313
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141220.13743620267333947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC84300.056.74231
TCTGTCG92350.055.461068
CTCGCTC82000.055.0780643
TCTCGCT89150.054.0558362
CGCTCTG79750.052.3911975
TCGCTCT97500.051.010054
TCACTCT149400.043.697694
GTCTCAC129000.043.6592181
GGCTAGA71450.042.1949921
GTATCAA190800.041.151051
CTGTCGC129850.039.535999
TCTGTCA130850.039.2334638
TAGAGTG74850.037.9011544
GCTCTGT195750.037.0192956
GGTATCA118750.036.8826561
ACGGGAC21100.036.8465423
GTACGGG438200.036.783821
CTAGAGT81550.036.169323
GCTAGAG81950.035.6306762
TCTCACT159950.035.5087662
Produced by FastQC (version 0.11.5)