FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6893711
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166920.24213373609656683No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG73520.10664792881511859No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG29950.047.041378
GGTATCA69100.046.305731
GTCTCGC27250.045.1787641
GTATCAA144650.045.1454931
CAACGCA151650.041.2593885
CTCGCTC28500.041.112513
ATCAACG153600.040.6197173
TCAACGC154350.040.6150134
TCTCGCT30900.040.2291072
AACGCAG162000.038.8216446
ACGCAGA161850.038.8203287
CGCAGAG163000.038.439838
CGCTCTG29500.038.3072555
GTCTCAC43050.037.3010521
GGCTAGA28100.032.594541
GCAGAGT193350.032.374989
GTACGGG281300.031.6083581
ACGGGAC15100.031.5112513
TATCAAC204000.031.4006162
TCTGTCA50600.029.2534628
Produced by FastQC (version 0.11.5)