FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894605
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555670.8059489992537644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323230.46881583498982177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244500.3546251018006108No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241130.34973722207436103No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77850.11291437290461165No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG76970.11163801262001231No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69220.10039733965905226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127200.069.8465041
GTATCAA296200.059.3468131
GTCTCGC26350.049.909551
TCTGTCG28700.049.7530148
ATCAACG357550.049.0806123
CAACGCA363650.048.434165
TCAACGC363850.048.3929334
AACGCAG377200.046.9100276
TATCAAC395250.044.609952
ACGCAGA402400.043.86857
CGCAGAG406050.043.5031558
CTCGCTC27700.042.75093
TCTCGCT30850.041.857812
GTACTTT557350.040.1236761
GCAGAGT443250.039.905829
TGCGGAC3300.039.664219
CGCTCTG27400.039.5243235
GTCTCAC45850.036.9894181
ACGGGAC14850.036.4658973
ACTTTTT618200.036.376413
Produced by FastQC (version 0.11.5)