FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894605
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182320.2644386444183532No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG76870.11149297167858058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC26950.052.0657461
TCTGTCG28200.049.9676178
CTCGCTC27600.046.1053053
GGTATCA74450.045.7602921
TCTCGCT30450.045.3057372
CGCTCTG27350.045.008445
GTATCAA145600.040.222931
ATCAACG154700.036.784753
CAACGCA154250.036.7022485
TCAACGC155850.036.325454
GTCTCAC44400.034.9507451
AACGCAG163050.034.645676
ACGCAGA163650.034.554487
ACGGGAC17050.034.526853
CGCAGAG164150.034.4452258
GTACGGG280100.031.3308471
GCTCTGT71450.030.9596
GGCTAGA25250.030.3757611
TCTGTCA50600.029.3750698
GCAGAGT195550.029.0639619
Produced by FastQC (version 0.11.5)