FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6895187
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552650.801501105046172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320640.46502002048675406No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244700.35488522646303866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241780.3506503884521188No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG78350.11362998566971426No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76930.111570578143856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132050.068.4606861
TCTGTCG28950.057.337948
GTATCAA289850.056.896441
GTCTCGC25750.048.7671171
ATCAACG354200.046.721253
TCAACGC358400.046.3231664
CAACGCA360950.045.9292955
AACGCAG373600.044.4307636
CTCGCTC28100.043.835513
CGCTCTG27300.043.1576925
TCTCGCT30050.042.7731552
TATCAAC388700.042.6815572
ACGCAGA403050.041.1843037
CGCAGAG406750.040.8242958
GTACTTT547800.039.9913141
ACGGGAC15350.038.7662353
GTCTCAC44650.037.5880241
CGCTAGA957.4653017E-637.5880241
CGTTACG957.473107E-637.58235
CTGTCGC44050.037.548149
Produced by FastQC (version 0.11.5)