FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6895187
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174880.25362618881837434No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG75690.10977222227620512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG29400.051.151018
GTCTCGC26150.050.485941
GGTATCA71550.045.1314541
CTCGCTC27450.045.066223
TCTCGCT29650.044.129092
CGCTCTG27800.043.0015955
GTATCAA146700.042.118661
CAACGCA153950.038.8257335
ATCAACG154950.038.7284133
TCAACGC157050.038.1729624
ACGCAGA162700.036.7544867
CGCAGAG162800.036.730318
AACGCAG163800.036.3987926
GTCTCAC45900.034.074711
ACGGGAC14700.033.9853743
GTACGGG278850.031.4138571
GCAGAGT192400.030.9556949
CTGTCGC49900.030.4942059
GGCTAGA26200.029.961381
TATCAAC203900.029.8974152
Produced by FastQC (version 0.11.5)