FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8056099
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700830.8699371743073168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416820.5173968194780129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345450.42880555464872017No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310520.3854471003894068No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA125350.155596399696677No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG119180.14793760602991596No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT119000.14771417282732002No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113240.14056431034424974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106760.13252071505079566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154450.081.17471
GTATCAA394000.062.8701021
ATCAACG506100.049.165633
TCAACGC515900.048.2316864
CAACGCA522600.047.6301885
AACGCAG544450.045.7724656
TATCAAC581850.042.959142
ACGCAGA585650.042.4711467
CGCAGAG588600.042.2886058
TCTGTCG10400.041.186148
GCAGAGT642050.038.814459
GCTCTAG13000.037.6045041
GTGGTAT61050.036.2291071
TGGTATC63600.035.3629572
GTACTTT689200.034.860141
ACTTTTT761600.032.1169623
AATCGCG750.00391473931.7285797
TACTTTT806900.030.3065172
AGAGTAC652000.029.75923310-11
GTCTCGC11950.029.4342821
Produced by FastQC (version 0.11.5)