FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8056099
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA423230.5253535240815685No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG131780.16357793021163222No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT113700.14113530630643936No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA105670.1311677028795202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82650.062.7599221
GTATCAA210100.054.659891
ATCAACG235000.047.8081553
TCAACGC240950.046.6281624
CAACGCA244050.046.108835
AACGCAG259100.043.4292266
ACGCAGA263500.042.6139377
CGCAGAG262700.042.6086858
TCTGTCG10100.039.370418
GCAGAGT303300.036.7098279
TATCAAC324650.034.9535832
GTACTTT116950.034.9141961
ACGGGAC28600.032.16513
GTCTCGC11100.030.4765451
GCTCTAG13150.028.433391
ACTTTTT140600.028.2396973
TCTCGCT12850.027.7119662
CAGAGTA306450.026.66868810-11
GTACGGG435500.026.3289031
AGAGTAC317050.026.07658210-11
Produced by FastQC (version 0.11.5)