FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8338703
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391960.4700491191495848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95550.051.2890851
GTATCAA193500.049.2083471
ATCAACG204500.045.0809333
TCAACGC208900.044.1314134
CAACGCA216050.043.2495575
AACGCAG228100.041.203756
CGCAGAG233400.040.0395978
ACGCAGA235750.039.614077
TCTGTCG26350.036.774358
TATCAAC257950.036.2238962
GCAGAGT271200.034.1075139
GTCTCGC28700.032.5346531
TCTCGCT33000.027.2155742
GTACGGG366300.027.1148991
CGCTCTG33100.026.2352165
ACGGGAC34100.026.1632333
AGAGTAC265250.025.96317510-11
GAGTACG277400.025.7278351
CAGAGTA282350.024.78025610-11
AGTACGG286850.024.5914632
Produced by FastQC (version 0.11.5)