FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8308060
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT652010.7847921175340573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408880.49214858823840946No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311250.37463619665722203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306500.36891885710984273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100200.12060577318892737No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96260.11586339049068012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163600.074.9965741
GTATCAA372600.059.518591
ATCAACG464650.048.880123
TCAACGC471500.048.2190174
CAACGCA482200.047.173155
TATCAAC500850.045.382852
AACGCAG503250.045.3163036
CGCAGAG542950.041.9587368
ACGCAGA543700.041.8463947
GCAGAGT591500.038.555029
TCTGTCG26400.035.60828
GTCTCGC27050.030.7961791
TGGTATC66050.030.7197482
GTACTTT616450.030.3376981
CAGAGTA596600.029.7386510-11
AGAGTAC587100.029.3432610-11
TCTCGCT29700.029.0500982
GTGGTAT71250.028.4777471
ACTTTTT720050.027.2784023
GTACGGG355550.027.1447751
Produced by FastQC (version 0.11.5)