FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8308060
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA414130.49846775300130236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91300.052.8844221
GTATCAA190450.050.7358471
ATCAACG205350.045.2624633
TCAACGC208500.044.552274
CAACGCA213750.043.374545
AACGCAG224850.041.3879976
CGCAGAG228200.040.749458
ACGCAGA232450.039.9578257
TCTGTCG26150.038.6525238
TATCAAC257550.036.5278472
GCAGAGT267150.034.5840269
GTCTCGC30050.033.441421
TCTCGCT31650.030.4383052
ACGGGAC32400.029.5498183
CGCTCTG31250.028.1647935
GTACGGG371500.027.7384551
GAGTACG278350.026.5962621
CTCGCTC35800.026.4111923
AGAGTAC260500.026.4050510-11
AGTACGG289050.025.3464362
Produced by FastQC (version 0.11.5)