FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8308838
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637000.7666535320582734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400980.4825945577468233No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302950.36461175437528087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301590.36297494306664785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99900.12023341892091288No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95210.11458882698158275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161100.072.994311
GTATCAA363900.059.2984471
ATCAACG455250.048.844813
TCAACGC465300.047.80264
CAACGCA476050.046.7225765
TATCAAC492950.045.036822
AACGCAG496200.044.9037746
ACGCAGA538850.041.3606837
CGCAGAG539000.041.338138
GCAGAGT588200.037.910759
TCTGTCG27500.036.779358
GTCTCGC29100.030.6757971
GTACTTT609350.029.3477781
AGAGTAC584100.029.19796410-11
CAGAGTA595900.028.9392810-11
TCTCGCT32600.028.8393042
CTCGCTC32450.028.055763
ACGGGAC32050.027.663273
TGGTATC69100.027.6421812
CGCTCTG33350.027.4767275
Produced by FastQC (version 0.11.5)