FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8339134
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT677050.8118948562284765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416570.4995362827842795No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319460.38308534195517185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305340.3661531281305709No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.12598430484508344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97660.11711048173587328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166150.074.019591
GTATCAA380900.060.6468851
ATCAACG475250.049.0146373
TCAACGC484150.048.1136174
CAACGCA491800.047.3600965
TATCAAC511700.045.639452
AACGCAG514050.045.5047386
ACGCAGA555800.042.1079647
CGCAGAG555800.042.065158
TCTGTCG24800.039.820338
GCAGAGT605350.038.651469
TGGTATC68700.033.690622
GTGGTAT73150.032.0277181
GTCTCGC26000.030.7241061
GTACTTT644750.030.4749931
AGAGTAC600050.029.61394310-11
ACGGGAC33200.029.3915123
CAGAGTA613950.029.2632410-11
TCTCGCT27850.028.8419592
ACTTTTT741300.026.944713
Produced by FastQC (version 0.11.5)