FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8339134
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460930.5527312548281392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81750.051.3931121
GTATCAA177650.049.7380641
ATCAACG188250.045.1416933
TCAACGC193700.043.9339034
CAACGCA201600.042.5655255
AACGCAG214550.039.9960826
CGCAGAG217000.039.3259668
ACGCAGA220400.038.907557
TCTGTCG28350.038.3070148
TATCAAC238750.036.289362
GTCTCGC27850.035.3705861
GCAGAGT256900.033.2651189
TCTCGCT29300.031.3930782
GTACGGG357400.027.8942151
CGCTCTG32450.027.0661985
GAGTACG266300.026.4285241
ACGGGAC32150.026.2106153
AGAGTAC247850.025.90825510-11
CTGTCGC42450.025.5837429
AGTACGG277000.025.2368622
Produced by FastQC (version 0.11.5)