FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7183446
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234540.3265006794789019No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG73170.10185919125723225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC30150.053.6613431
TCTGTCG31850.050.399748
CTCGCTC28400.050.0591853
TCTCGCT32100.048.180632
ACGGGAC17200.044.959253
CGCTCTG30900.044.4693455
GGTATCA70500.044.2945331
GTATCAA150550.041.8403321
GTACGGG324800.039.7029881
CAACGCA153450.038.8038145
GAGTCGG8600.038.040321
ATCAACG158600.037.5810363
TGCGGAC4000.037.158149
ACGCAGA160600.037.05537
TCAACGC161150.036.986364
CGCAGAG160700.036.8482978
AACGCAG162050.036.6154636
GTCTCAC50200.036.1389961
ACGGGTC6450.035.0451623
GCAGAGT171850.034.2499249
Produced by FastQC (version 0.11.5)