FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7167260
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT548030.7646297190279131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341360.4762768477772538No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250830.34996637487687066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228040.31816900740310805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82460.11505093996869098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137200.072.515671
TCTGTCG32500.060.0466358
GTATCAA310100.057.144261
GTCTCGC30250.056.848911
CTCGCTC29400.052.8254173
TCTCGCT31000.052.402342
ACGGGAC16650.049.676423
CGCTCTG29350.048.8588835
ACGGGTC6350.047.7910043
ATCAACG379000.046.363263
CAACGCA380700.046.1077235
TCAACGC385250.045.6413544
AACGCAG396300.044.4851346
GTACGGG312700.042.47331
ACGCAGA416650.042.1404387
GTCTCAC48650.042.0751151
GGCTAGA29100.041.918971
TATCAAC420900.041.9175072
CGCAGAG418900.041.9138038
CGGGAAC9950.040.6658134
Produced by FastQC (version 0.11.5)