FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7167260
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249610.3482641902205306No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG76390.10658187368673662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC29900.053.298691
TCTCGCT31400.049.052652
TCTGTCG33400.048.9570128
CTCGCTC29800.048.093583
GGTATCA72600.047.341741
ACGGGAC16900.045.041163
CGCTCTG31650.043.5925945
GTATCAA153750.042.4272081
ACGGGTC7100.041.879163
CAACGCA157350.040.0624585
GAGTCGG9050.038.766481
ATCAACG163400.038.760013
GTACGGG320850.038.7344321
ACGCAGA164600.038.475557
GTCTCAC47950.038.443781
CGCAGAG165500.038.2271848
AACGCAG165550.038.2193766
TCAACGC165750.038.139784
TGCGGAC3400.036.723059
GGCTAGA28700.035.8440551
Produced by FastQC (version 0.11.5)