FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7175126
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT548110.7639029614253464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340770.4749324262737686No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249100.3471716036763675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223760.31185515069700515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83550.1164439481620253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134350.074.5941541
TCTGTCG32450.058.4876758
TGCGGAC2750.058.414349
GTATCAA310000.057.8412671
GTCTCGC28850.051.3634151
CTCGCTC28100.048.4918753
ATCAACG370250.048.100713
CAACGCA373050.047.7077835
TCTCGCT30500.047.4074132
TCAACGC376200.047.3399434
AACGCAG386500.046.180666
ACGGGAC14250.045.097113
ACGGGTC5750.044.4979973
ACGCAGA408100.043.73647
CGCTCTG29800.043.7288135
CGCAGAG410500.043.553168
TATCAAC414300.042.9864542
GTACGGG309550.041.8723641
GTCTCAC49900.041.6222231
GCAGAGT433300.041.275159
Produced by FastQC (version 0.11.5)