FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7186625
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537140.7474162071904406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332880.46319378011236156No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228670.31818830118449204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217710.3029377489433496No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81690.11366949019880682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138350.072.9131851
TCTGTCG31850.060.8908928
GTATCAA310700.057.8152921
GTCTCGC32400.056.306851
TCTCGCT33100.053.746572
CTCGCTC31450.053.5393833
CGCTCTG31900.050.7264485
ATCAACG377500.047.5359273
ACGGGAC15200.047.364113
CAACGCA382400.046.9212575
TCAACGC383250.046.8382574
AACGCAG397050.045.503396
ACGGGTC6050.045.2386933
ACGCAGA422100.042.7888457
TATCAAC419950.042.759172
CGCAGAG424350.042.5900048
TGCGGAC3550.041.8943829
TAACGCA3900.041.1914184
GTACGGG310550.041.045071
GCAGAGT444000.040.7051129
Produced by FastQC (version 0.11.5)