FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7186625
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA284690.39613866035865236No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG72320.10063138121162576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG30600.053.9146548
GTCTCGC30200.051.6782881
CTCGCTC29600.050.320433
TCTCGCT31200.048.8810842
CGCTCTG30450.047.163775
GGTATCA68850.046.9733851
ACGGGAC16600.041.467923
GTATCAA145300.041.2083171
GTACGGG327400.039.5852361
TGCGGAC2850.039.5638249
CAACGCA150350.038.0894175
GTCTCAC49350.037.0358961
ACGGGTA10800.036.814033
ATCAACG157500.036.5094683
CGCAGAG157200.036.4676748
AACGCAG156950.036.4493756
ACGCAGA157350.036.3190047
TCAACGC158950.036.1032564
GAGTCGG8950.035.140841
GGCTAGA31250.034.1807631
Produced by FastQC (version 0.11.5)