FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6245381
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262390.42013449619807025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188190.3013266924788095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137300.2198424723807883No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67860.10865630135295189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG5150.083.1808241
GGTATCA84550.071.5657651
TCTGTCG41800.054.652218
GTATCAA196600.053.8987051
TGCGGAC2950.052.4329269
TCTCGCT41350.049.9260942
CTCGCTC39050.048.7531363
AGCGCGA4100.047.8881451
GTCTCGC37150.047.7260441
CGCTCTG37850.047.4695055
ATCAACG268600.046.204253
CAACGCA270050.045.978195
TCAACGC273350.045.4666564
ACGGGAC12750.044.7955253
AACGCAG280350.044.5644876
TATCAAC287600.043.2552452
ACGCAGA303100.041.160697
CGCAGAG305250.040.966598
ATACGGG37950.040.919161
GCAGAGT321350.038.932639
Produced by FastQC (version 0.11.5)