FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6245381
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77670.12436390990397544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC37850.061.118251
TCTGTCG43000.055.994898
TCTCGCT40650.055.74252
CTCGCTC37350.055.4128763
GGTATCA61600.053.289981
CGCTCTG39000.049.4085855
GTATCAA122300.046.8259931
GTCTCAC63150.042.9416431
CAACGCA132650.042.458515
GTACGGG276250.041.6118241
TCGCTCT54550.041.102544
ACGGGAC14000.040.781693
AACGCAG139250.040.7287456
ACGCAGA139250.040.7280967
ACGGGTC9500.040.6922573
CGCAGAG140400.040.523478
ATCAACG142800.039.5239223
TCAACGC145100.039.0203864
TCTGTCA64000.039.0149578
GGCTAGA32500.037.5109181
Produced by FastQC (version 0.11.5)