FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6207533
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260930.4203441206031446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187510.30206847067909265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133990.21585064469250506No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70100.11292730944805288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG5450.081.883211
GGTATCA87000.069.760731
TCTGTCG41750.059.56738
TCTCGCT39600.054.2427982
CTCGCTC37500.053.3136063
GTCTCGC33850.052.0311131
CGCTCTG37750.050.1213465
ACGGGAC13350.049.919113
GTATCAA190750.049.4106641
AGCGCGA4250.049.001481
TAACGCA1850.048.2427564
ATCAACG261950.042.2270773
CAACGCA262700.042.2180755
ATACGGG37100.042.1805231
TCAACGC266700.041.5179524
TCGCTCT51950.041.1170044
AACGCAG271550.041.0157976
TGCGGAC3350.040.8480349
GTCTCAC57400.040.8426971
TATCAAC274750.040.21652
Produced by FastQC (version 0.11.5)