FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6207533
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82060.1321942227290616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG43000.056.5532048
CTCGCTC38300.056.2032283
GTCTCGC40550.056.1614531
GGTATCA59200.055.443141
TCTCGCT42000.054.084032
CGCTCTG41600.049.4613275
GTATCAA122250.048.2981831
GTCTCAC61150.043.7568931
CAACGCA132750.043.3634535
ACGGGTC8550.042.4243473
TCGCTCT54750.042.3607334
GTACGGG274300.041.7937771
AACGCAG140350.041.3509376
ACGCAGA140050.041.311477
TGCGGAC3500.040.7688149
CGCAGAG141800.040.7141538
ATCAACG142850.040.544333
GGCTAGA34550.040.443641
TCAACGC144050.040.2095034
ACGGGAC14350.040.1949353
Produced by FastQC (version 0.11.5)