FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175912
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247890.40138201451056943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176380.2855934475750302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128410.20792070871476145No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76460.12380357751211479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG7400.082.8303451
GGTATCA80300.068.846861
TCTGTCG42950.060.535118
CTCGCTC35700.053.999153
TCTCGCT39300.051.929212
GTATCAA188100.051.8213961
CGCTCTG36950.050.884185
GTCTCGC33450.050.8807951
AGCGCGA3850.049.462181
ATACGGG40100.048.0822331
ACGGGAC11400.046.4510233
ATCAACG261450.045.491923
CAACGCA260500.045.315225
TCAACGC265550.044.7447284
AACGCAG271200.043.678796
ACGTACG558.524846E-443.2794041
TATCAAC275450.042.8773422
ATACGGC1151.4259058E-841.3976941
TCGCTCT51550.041.204984
CTGTCGC63750.040.690729
Produced by FastQC (version 0.11.5)