FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175912
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80930.13104137494187093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.053.6703221
GTCTCGC39600.052.8607641
TCTGTCG44100.052.844498
CTCGCTC38000.050.5526123
TCTCGCT42000.050.4101942
GTATCAA121700.048.5715261
CGCTCTG40700.045.5927125
TGCGGAC3350.044.3642739
CAACGCA130050.044.269065
ACGCAGA136050.042.8243067
AACGCAG136350.042.4681246
CGCAGAG136600.042.4332358
GTCTCAC63250.042.2154851
ACGGGAC13600.041.981413
ATCAACG139350.041.697723
GGCTAGA33750.041.58381
TCAACGC140750.041.1149444
GTACGGG273000.041.0397031
TCGCTCT55800.039.650074
TACGGGT44100.039.109032
Produced by FastQC (version 0.11.5)